Non-Coding RNA Extraction from Nucleus Pulposus of Intervertebral Discs with Subsequent Expression Profiling
Background.With numerous extraction protocols for total RNA and RNA fractions, like microRNA (miRNA) and long non-coding RNA (lncRNA), available for various cell and tissue types, obtaining a high quantitative and qualitative yield from some special material, such as the nucleus pulposus, remains challenging.Nucleus pulposus is troublesome to manag